Immunopeptidomics:Article Title: FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells
Article Snippet: .. Target Label Clone Specificities Source Cat # Dilutions CD45 (Ms) 89Y 30-F11 Ms DVS-Fluidigm 3089005B 1μl/100μl CD4 (Ms) 115In RM4-5 Ms BioLegend 100506 1μl/100μl Ly-6G/Ly-6C, Gr-1 139La RB6-8C5 Ms BioLegend 108402 1μl/100μl Ly-6G 141Pr 1A8 Ms DVS-Fluidigm 3141008B 1μl/100μl CD39 142Nd 24DMS1 Ms DVS-Fluidigm 3142005B 1μl/100μl CCR2 143Nd 475301 Ms R&D Systems MAB55381 1μl/100μl CD115 144Nd AFS98 Ms DVS-Fluidigm 3144012B 1μl/100μl CD68 145Nd FA-11 Ms BioLegend 137002 1μl/100μl CD8a (Ms) 146Nd 53-6.7 Ms DVS-Fluidigm 3146003B 1μl/100μl CD223, LAG- 3 147Sm C9B7W Ms BioLegend 125202 1μl/100μl CD11b (Ms) 148Nd M1/70 Ms DVS-Fluidigm 3148003B 1μl/100μl CD19 (Ms) 149Sm 6D5 Ms DVS-Fluidigm 3149002B 1μl/100μl CD25 150Nd 3C7 Ms BioLegend 101902 1μl/100μl TIGiT, Vstm3 151Eu 1G9 Ms BioLegend 142102 1μl/100μl CD3, CD3e 152Sm 145-2C11 Ms BioLegend 100302 1μl/100μl CD182, CXCR2 153Eu SA044G4 Ms BioLegend 149302 1μl/100μl CD274, PD- L1 154Sm 10F.9G2 Ms BioLegend 124303 1μl/100μl CD69 156Gd H1.2F3 Ms BioLegend 104533 1μl/100μl Foxp3 158Gd FJK-16s Ms, Rt, Bv, Cn, Po, Fe DVS-Fluidigm 3158003A 1μl/50μl CD279, PD-1 159Tb 29F.1A12 Ms BioLegend 135202 1μl/100μl CD62L 160Gd MEL-14 Ms DVS-Fluidigm 3160008B 1μl/100μl CD183, CXCR3 161Dy CXCR3-173 Ms BioLegend 126502 1μl/100μl Ly-6C 162Dy HK1.4 Ms DVS-Fluidigm 3162014B 1μl/100μl CD152, CTLA-4 163Dy 9H10 Ms BioLegend 106202 1μl/100μl CD49b 164Dy HMa2 Ms DVS-Fluidigm 3164011B 1μl/100μl IFNg 165Ho XMG1.2 Ms DVS-Fluidigm 3165003B 1μl/100μl CD44 166Er IM7 Hu, Ms, Ch, Rh BioLegend 103002 1μl/100μl Ki67 168Er B56 Ms, Hu BD 556003 1μl/50μl CD206, MMR 169Tm C068C2 Ms DVS-Fluidigm 3169021B 1μl/100μl CD127, IL- 7Ra 170Er A7R34 Ms BioLegend 135029 1μl/100μl CD80 171Yb 16-10A10 Ms, Cn DVS-Fluidigm 3171008B 1μl/100μl CD86 172Yb GL1 Ms DVS-Fluidigm 3172016B 1μl/100μl F4/80 173Yb BM8 Ms BioLegend 123102 1μl/100μl I-A/I-E MHC- II 174Yb M5/114.15.2 Ms DVS-Fluidigm 3174003B 1μl/100μl CD28 175Lu 37.51 Ms BioLegend 102119 1μl/100μl CX3CR1 176Yb SA011F11 Ms BioLegend 149002 1μl/100μl CD11c 209Bi N418 Ms DVS-Fluidigm 3209005B 1μl/100μl Abbreviations: Ms, mouse; Rt, rat; Bv, bovine; Cn, canine; Po, porcine; Fe, feline; Hu, human; Ch, Chicken; Rh, rhesus Supplementary Table 5. .. Antibodies used for Western blotting, Immunohistochemistry and Immunofluorescence Target Clone Specificities Source Cat # Dilutions FGL2 #4 Ms/Hu MD Anderson Monoclonal Antibody Core Facility 1:500 for WB 1:250 for IHC, IF β-Actin 13E5 Hu/Ms/Rt/Mk Cell Signalling Technology 5057 1:1000 GAPDH D16H11 Hu/Ms/Rt/Mk Cell Signalling Technology 5174 1:1000 PIDD Anto-1 Ms/Hu Novus Biologicals, Inc NBP1-97595 1:500 CD3 SP7 Ms/Hu/Rt Thermo Fisher scienctific MA1-90582 1:350 CD90.1 HIS51 Ms/Rt Thermo Fisher scienctific 14-0900-81 1:1000 CD90.2 53-2.1 Ms Thermo Fisher scienctific 14-0902-82 1:500 Histag J095G46 Ms/Hu/Rt BioLegend 362618 1:1000 IgG Rab Thermo Fisher scienctific A-21245 1:1000 IgG Ms Thermo Fisher scienctific A-11063 1:1000 IgG Rt Thermo Fisher scienctific A-11077 1:1000 Abbreviations: Ms, mouse; Rt, rat; Hu, human; MK, monkey; Rab, Rabbit; WB, western blotting; IHC, immunohistochemistry; IF, immunofluorescence.
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